Background: Febrile seizure is one of the cause for convulsion among children and is common in the age-groups of 1–5 years, with nearly 4% having minimum one episode of febrile convulsion. Educating mothers on the nature of the febrile seizure and home care is the most important aspect of the management. Video-assisted teaching is an awareness program for mothers of children aged under five on prevention and management of febrile seizure. Conceptual model used for the study was general system theory (Ludwig von Bertalanffy-1968). Objectives: To assess the level of knowledge on management and prevention of febrile seizure among mothers of children aged under five and evaluate the effectiveness of video-assisted teaching on management and prevention of febrile seizure and associate the level of knowledge on management and prevention of febrile seizure with selected demographic variables of mothers with children aged under five. Materials and methods: The research approach was quantitative, the research design was preexperimental, a one group pre- and posttest design was used for the study. A sample was 100 mothers with children aged under five were selected through nonprobability convenient sampling method on knowledge regarding management and prevention of febrile seizure. It was assessed by structured questionnaire, where during the pretest the mothers of children aged under five were attended the video-assisted teaching regarding management and prevention of febrile seizure. Posttest data were collected after 1 week of implementation of video-assisted teaching. Results: The study results show that video-assisted teaching on febrile seizure among mothers of children aged under five was statistically highly significant at p value <0.001 level. Conclusion: The video-assisted teaching was effective and improved the knowledge regarding management and prevention of febrile seizure among mothers of children aged under five.