Statement of the problem: A study to assess the effectiveness of a structured teaching program on knowledge regarding non-invasive diagnostic procedures used to assess fetal well-being among staff nurses at a selected hospital, Coimbatore. Objectives: A) To assess the level of knowledge regarding non-invasive diagnostic procedures used to assess the fetal well-being among staff nurses. B) To evaluate the effectiveness of a structured teaching program on knowledge regarding non-invasive diagnostic procedures used to assess the fetal well-being among staff nurses. C) To find out the association between the level of knowledge with selected demographic variables. Materials and methods: A quantitative approach one group pre-test post-test design was used for the study. A total of 60 samples were selected by using a convenient sampling technique. The pre-test level of knowledge among staff nurses was assessed using a structured questionnaire. The structured teaching program was administered after which post-test was conducted on the 7th day using the same questionnaire to evaluate the effectiveness of the structured teaching program. The obtained data was analyzed using descriptive inferential statistics. Results: The mean pre-test knowledge score was 17.28 whereas the mean post-test knowledge score was 26.73. The obtained “t-value” (25.97) for knowledge was higher than the table value [significant at (0.05) level]. The result reveals significant improvement in the level of knowledge after the teaching program. Conclusion: This study shows the structured teaching program was effective in improving the nurse's knowledge on non-invasive diagnostic procedures.